Fig. 1.

TB degradation of 33E2 and 54E5 clones. a Schematic diagram of TB esterase screening of BAC clones. b Representative images of a clear zone formation around TB degrading positive clones, 33E2 and 54E5. E. coli DH10B containing pIndigoBAC-5 (vector-only control) is also shown as a control. c Measurement of butyrate production by 33E2 and 54E5 in the presence of 20 mM of TB. Three independent experiments were performed, and values (means ± SEM) are displayed in each bar. **, P < 0.05 compared to Control