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. 2021 Mar 6;40:90. doi: 10.1186/s13046-021-01880-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — METTL3 triggered enhanced FBXW7 mRNA translation. a FBXW7 mRNA was analyzed by RT-PCR in HCC827 cells with METTL3 overexpression or depletion. b Nuclear and cytoplasmic FBXW7 mRNAs were extracted separately. c FBXW7 mRNA was analyzed in HCC827 cells transfected with METTL3 control or shRNA plasmids after ActD treatment. d Western blot analysis of the cytoplasmic and nuclear fractions of HCC827 cells using polyclonal antibodies against METTL3, METTL14, WTAP, YTHDF1/2/3, LaminB (a nuclear protein), and α-tubulin (a cytoplasmic protein). e FBXW7 protein expression was determined by western blot analysis in HCC827 cells treated with CHX to block protein synthesis. f HCC827 cells were pretreated with CHX or MG-132 and then treated with control vectors or METTL3 plasmids. g Firefly (F-Luc) values were normalized against Renilla luciferase levels, and FBXW7 translation efficiency was calculated for the pmirGLO-FBXW7 reporter relative to pmirGLO in METTL3 knockdown and control HCC827 cells. Bar = mean ± SD. n.s., not significant; **p < 0.01