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. 2021 Mar 1;9:e10874. doi: 10.7717/peerj.10874

Table 4. Expressivity of multifoliolate trait (%) in recurrent cultures of axillary buds of Trifolium alexandrinum.

Culture in different media
Source plant (Explants type) 1 C 2 SC 3SC 4 SC 5 SC 6 SC 7SC 8 SC 9 SC Average (%)
LSP3 RL RL LSP3 MS LSP3 RL RL LSP3 LSP3 RL
Wardan-Trifoliolate (T) Tri 100.0 100.0 100.0 100.0 100.0 100.0 96.4 100.0 99.6
Quadri 0.0 0.0 0.0 0.0 0.0 0.0 2.4 0.0 0.3
Penta 0.0 0.0 0.0 0.0 0.0 0.0 1.2 0.0 0.2
Penta 1-Pentafoliolate (P) Tri 9.0 5.8 4.2 4.7 14.1 11.9 14.2 26.6 22.7 0.0 0.0 10.3
Quadri 11.8 7.2 3.1 9.7 3.1 10.5 15.7 22.5 6.8 1.9 0.0 8.4
Penta 79.2 86.3 92.6 85.6 76.3 77.6 70.1 50.9 70.5 98.1 100.0 80.7
Hexa 0.0 0.7 0.0 0.0 5.0 0.0 0.0 0.0 0.0 0.0 0.0 0.5
Hepta 0.0 0.0 0.0 0.0 1.6 0.0 0.0 0.0 0.0 0.0 0.0 0.1
Penta 1-Multifoliolate (P) Tri 33.8 26.4 30.6 30.9 31.7 52.0 42.6 55.3 45.6 17.9 36.7
Quadri 17.1 11.1 15.1 5.5 12.0 17.1 22.2 20.0 14.0 0.0 13.4
Penta 47.3 59.6 52.5 63.6 56.3 30.2 34.6 24.4 40.4 72.1 48.1
Hexa 1.6 2.5 1.2 0.0 0.0 0.7 0.3 0.3 0.0 10.0 1.7
Hepta 0.2 0.4 0.6 0.0 0.0 0.0 0.2 0.0 0.0 0.0 0.1
Penta 1-Multifoliolate (Q) Tri 33.0 17.1 22.1 48.6 42.8 40.6 61.5 38.0
Quadri 13.8 16.4 23.5 5.4 26.1 14.6 23.1 17.6
Penta 49.1 54.0 42.3 34.9 29.7 44.9 15.4 38.6
Hexa 3.0 10.6 12.0 11.1 1.4 0.0 0.0 5.4
Hepta 1.0 1.9 0.0 0.0 0.0 0.0 0.0 0.4
Penta 1-Multifoliolate (T) Tri 47.1 35.3 52.2 36.4 50.0 61.5 51.4 47.4 54.8 0.0 0.0 39.6
Quadri 22.9 17.9 5.6 9.1 21.4 11.4 20.9 31.2 36.4 7.7 0.0 16.8
Penta 30.0 46.9 42.2 54.6 28.6 27.1 27.7 21.5 8.8 92.3 100.0 43.6
Penta 1-Trifoliolate (T) Tri 56.5 47.7 67.6 61.9 95.5 79.8 96.0 13.0 18.2 59.6
Quadri 26.1 26.4 19.7 7.1 2.3 11.5 2.8 4.4 10.5 12.3
Penta 17.4 25.9 12.7 31.0 2.3 8.8 1.3 82.6 71.3 28.1

Notes.

C
Culture
SC
Subculture
Tri
Trifoliolate
Quadri
Quadrifoliolate
Penta
Pentafoliolate
Hexa
Hexafoliolate
Hepta
Heptafoliolate

2SC, 3SC,... denotes cycle of sub-culture.