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. 2021 Feb 9;27(3):gaab009. doi: 10.1093/molehr/gaab009

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© The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — DNAH8 is present in sperm flagella of DNAH5-deficient individuals. A, IF analysis showing DNAH8 panaxonemal localization in human control sperm cells. B–D, same staining procedure shows that sperm flagella of DNAH5-mutant individuals present an unaltered DNAH8 localization along the entire flagellar length. This is consistent with the observed unaltered sperm ultrastructure of DNAH5-deficient individuals and the hypothesis that DNAH8, as a paralogue of DNAH5, developed a specific function in sperm flagella. Flagellar marker: acet. tubulin (acetylated alpha-tubulin). Nuclei (blue) were stained with DAPI. Scale bars represent 10 µm.