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. 2021 Feb 25;10:e62312. doi: 10.7554/eLife.62312

Figure 6. Non-stop maintains the protein level and intranuclear localization the NIC subunits, (A–H).

(A–H) Representative confocal images of the midgut tissue using the indicated antibodies (red) and expressing the indicated transgenes in EC using the MyoIA-Gal4/Gal80ts system. UAS-LacZ (A, C, E, G), UAS-Non-stop RNAi (B, D, F, H). DAPI marks DNA (blue), and scale bar is 10 μM. White arrows points to cells shown in insets, and scale bar is 10 μM. (I–L) Quantification of 3 biological experiments is shown (M–T) Non-stop regulate large-scale organization of the nucleus. Representative confocal images of the midgut tissue using the indicated antibodies (red) and expressing the indicated transgenes in EC using the MyoIA-Gal4/Gal80ts system. UAS-LacZ (M–P), UAS-Not RNAi (Q–T).

Figure 6—source data 1. Quantification of cell populations described in 6I-L.

Figure 6.

Figure 6—figure supplement 1. NIC subunits are required for maintaining large-scale organization of the EC nucleus.

Figure 6—figure supplement 1.

(A–O) Confocal images of the midgut tissue using the indicated antibodies (red) and expressing the indicated transgenes in EC using the MyoIA-Gal4/Gal80ts system, DAPI marks DNA (blue) and scale bar is 10 μM M (A, D, G, J, M) Mtor; (B, E, H, K, N) Coilin (C, F, I, L, O) Nop60B; (A–C) Control, (D–F) UAS-Cp190 RNAi; (G–I) UAS-e(y)2 RNAi (J–L) Mod(Mdg4) RNAi (M–O) Nup98-96 RNAi.