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. 2021 Mar 2;14:1543–1552. doi: 10.2147/OTT.S298576

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© 2021 Zhang et al.

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Circulating tumor cell (CTC) subpopulations and their relationship with survival. CTCs were stained for epithelial markers (EpCAM and CK8/18/19, red fluorescence) and mesenchymal markers (Vimentin and Twist, green fluorescence) and classified as epithelial CTCs (E-CTCs) (A), epithelial/mesenchymal hybrid CTCs (E/M-CTCs) (B) or mesenchymal CTCs (M-CTCs) (C). Comparison of CTC counts in patients stratified by alpha-fetoprotein (AFP) level (D), tumor number (E), hepatitis B virus (HBV) DNA (F), and Barcelona Clinic Liver Cancer (BCLC) stage (G). Difference analysis were assessed for significance using the Pearson chi-squared test.