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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Mass Spectrom Rev. 2020 Sep 7;41(2):158–177. doi: 10.1002/mas.21653

Figure 2|. Middle-down FT-ICR MS characterization of 223-kDa human cardiac myosin heavy chain using combined limited digestion strategies.

Figure 2|

A) Schematic showing complete sequence coverage for human β-MHC achieved by middle-down MS with limited digestion with Glu-C (blue), Asp-N (red), and trypsin (green). Peptide mapping is based on accurate mass measurements and MS/MS experiments B) Localization of trimethylation (Lys129) on polypeptide D[74-217] and methylation (Lys34) on polypeptide A[11-73] from Glu-C digestion of human β-MHC. Reprinted and adapted with permission from Jin, Y., Wei, L., Cai, W., Lin, Z., Wu, Z., Peng, Y., Kohmoto, T., Moss, R., and Ge, Y. Anal. Chem. 2017. 89, 4922-4930 (Jin et al., 2017). Copyright © 2017 American Chemical Society.