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. 2021 Mar 7;42(6):1067–1074. doi: 10.1016/j.rbmo.2021.03.005

Figure 2.

Figure 2

Validation of the multiplex RT-qPCR assay. TaqMan assay containing primers and probes for lentivirus (LV, red), and SARS-CoV-2 (N1N2, blue; ORF1ab, orange) was mixed in an optimized concentration to prepare the multiplex RT quantitative PCR (RT-qPCR) assay. Amplification of 100, 20 and 4 copies of lentivirus genome (A), SARS-CoV-2 genome (B), a mixture of 100 copies of both SARS-CoV-2 and lentivirus genome (C), and the RT-qPCR negative control (D) using multiplex RT-qPCR. Each experiment was repeated three times independently, and the reaction was performed in technical duplicates. Red squares depict the baseline start, and green squares depict the baseline end, of each well.