Figure 3.

Multiplex RT quantitative PCR (RT-qPCR)-based SARS-CoV-2 screening of IVF samples. Representative image of multiplex RT-qPCR amplification with RNA isolated from follicular fluid (A), vitrification solution (B), and culture medium (C) collected during patient IVF treatment cycles (n = 50 for each sample category shown). All of the samples showed amplification of the external positive control (lentivirus) and no amplification of N1/N2 (blue) and ORF1ab (orange) of the SARS-CoV-2 loci. (D) Multiplex RT-qPCR of the positive control showed successful amplification of all the target loci. Each reaction was performed in technical duplicates. Red squares depict the baseline start, and green squares depict the baseline end, of each well.