Table 1.
Value | PacBio | PacBio + 10X | Final Assembly |
---|---|---|---|
Scaffolds | 6,770 | 6,591 | 2,368 |
Contigs | 6,770 | 6,877 | 2,472 |
Total length | 1,847,057,843 | 1,860,819,131 | 1,776,755,624 |
Scaffold N50 | 1,241,163 | 1,977,022 | 2,051,244 |
Scaffold N75 | 570,763 | 915,592 | 1,056,644 |
Scaffold L50 | 436 | 265 | 245 |
Scaffold L75 | 981 | 608 | 546 |
N’s per 100 kbp | 0 | 0.55 | 0.53 |
Complete single copy | 892 (93.5%) | 891 (93.4%) | 894 (93.7%) |
Complete duplicated | 11 (1.2%) | 12 (1.3%) | 9 (0.9%) |
Fragmented | 10 (1.0%) | 10 (1.0%) | 11 (1.2%) |
Missing | 41 (4.3%) | 41 (4.3%) | 40 (4.2%) |
Note.—The three steps columns represent the polished assembly derived from wtdbg2 (PacBio), the polished assembly generated from the Tigmint+ARKS pipeline (PacBio + 10X), and the final assembly after removing highly heterozygous haplotypes (final assembly).