Figure 2.

Twenty-four hours growth assays to assess general metal and antibiotic resistance associated with FeSO₄ adaptation. Twenty-four hours optical assays were constructed similarly to those in Fig. 1 in seven antibiotics with varying mechanisms of action. (A) Ampicillin, (B) bacitracin, (C) polymyxin-B, (D) rifampin, (E) tetracycline (F) chloramphenicol and (G) sulfanilamide. All iron (II)-adapted populations showed increased resistance to four of the seven antibiotics tested. This data demonstrates the pleiotropic effects associated with iron (II) adaptation which leads to general antibiotic resistance. Statistical analysis was performed identically to Fig. 1 with both the F-statistic and P-values are displayed Table 1