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Mechanism of Pue in regulating mitochondrial autophagy through SIRT-1. (a–e) Changes in transcription of atg3, atg7, sirt-1, and PINK1/parkin were determined by qPCR. ∗p < 0.05. (f) Mitochondrial autophagy was observed with transmission electron microscopy. (g, h) Protein expression of Sirt-1, LC3-I\II, Beclin-1, and Bcl-2 was measured using western blotting. ∗p < 0.05.
