Fig. 11.

Time and Dose Dependent Increase of FUBP1 Association with Ribosomes. Hela cells were treated with 100 μM H₂O₂ for 10 min before harvesting at indicate time points (A) or were treated with various doses of H₂O₂ for 10 min before harvesting at 60 min after (B). Cells were lysed for total ribosome isolation using sucrose cushion and ultracentrifugation as described in the methods. Ribosomal proteins were resolved by SDS-PAGE for Western blot to detect FUBP1 protein using S6 as a loading control. The bar graphs showed average ± SD of FUBP1 band intensity over that of corresponding loading control from three independent experiments. * indicates significant different between control versus the H₂O₂ treated group (p < 0.05).