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Presence of FUBP1 in the 40/43S Ribosomal Fraction. Hela cells were treated with 100 μM H2O2 for 10 min and harvested 1 hour after for isolation of ribosomes and separation of ribosomal subunits using a fractionator as described in the Methods. The proteins collected in the fractions between 8 and 21 min were used for Western blot to detect indicated proteins.
