Fig. 13.

Immunoprecipitation of FUBP1 for Detection of eIFs. Hela cells were treated with 100 μM H₂O₂ for 10 minutes and lysed in RIPA buffer 1 hour after for immunoprecipitation. FUBP1 was captured and pulled down from cell lysate by anti-FUBP1 antibody and agarose-protein A/G beads. Mouse IgG was used as a control for immunoprecipitation. Protein aliquots from total cell lysates were used as input or loading control. Immunoprecipitated proteins and input were resolved by SDS-PAGE gel for detection of binding proteins as indicated.