Fig. 8.

Lack of Time Dependent Nuclear to Cytoplasmic Translocation of FUBP1. Hela cells were treated with 100 μM H₂O₂ for 10 minutes and subsequently cultured in fresh DMEM containing 0.5% FBS for indicated time point before harvesting for total cell lysates or fractionation into cytosolic and nuclear extracts. The level of FUBP1 protein was determined by Western Blot. Lamin B1 was used as a loading control for nuclear fraction, whereas GAPDH was used as a loading control for cytosolic or total proteins. The bar graphs represent average ± SD of FUBP1 band intensity over that of corresponding loading control from three independent experiments. * indicates statistically significant difference between control versus the H₂O₂ treated group (p < 0.05).