Table 3.
Mass-specific respiration rates in the critically ill and reference cohorts.
| JO2 per mass (pmol/s/mg wet weight) median (IQR) | Reference group (n = 9) | Critically ill patients < 48 h (n = 21) | Critically ill patients day 5–7 (n = 12) |
|---|---|---|---|
| LEAKFAO | 5.49 (4.82–6.75) | 4.55 (3.17–5.51) * | 4.54 (3.62–6.02) |
| OXPHOSFAO | 14.7 (10.5–17.1) | 9.96 (7.16–10.8) ** | 9.25 (7.20–13.4) * |
| OXPHOSCI | 26.2 (19.6–31.3) | 20.5 (17.3–23.5) | 26.8 (22.0–30.8)## |
| OXPHOSMAX | 44.6 (38.4–59.4) | 44.8 (38.0–53.2) | 47.5 (45.9–58.2) |
| ETSMAX | 53.0 (45.8–71.8) | 55.59 (48.0–66.6) | 62.6 (57.5–71.5)## |
| ETSCII | 24.3 (21.0–32.9) | 24.5 (22.5–27.8) | 25.5 (22.2–32.3) |
Respiratory capacities generated by sequential addition of substrates, uncouplers and inhibitors according to previously described SUIT protocol. JO2: oxygen consumption rate at steady state; LEAKFAO: capacity for respiration supported by proton leak, in the presence of malate and octanoyl carnitine and absence of ADP; OXPHOSFAO: capacity for oxidative phosphorylation supported by FAO, following addition of saturating ADP; OXPHOSCI: capacity for oxidative phosphorylation supported by complex I; following addition of saturating pyruvate and glutamate; OXPHOSMAX: maximum capacity for oxidative phosphorylation supported by complex I and complex II, following addition of succinate; ETSMAX: maximum capacity of the electron transport system uncoupled from oxidative phosphorylation, following titration of uncoupler; ETSCII; capacity of uncoupled electron transport system supported by complex II alone, following inhibition of complex I by rotenone. *p < 0.05, **p < 0.01 vs reference group; #p < 0.05, ##p < 0.01 vs critically ill patients within 48 h.