Figure 5.

Selective suppression of polyQ-expanded androgen receptor by i.c.v.-injected LUNAR-REPU910 siRNA

(A) Scheme of the experiment. LUNAR-EGFP mRNA (1,800 ng) complexes or vehicle were i.c.v. injected into AR24Q or AR97Q mice at P1. At P4, mice were sacrificed, and their brains were dissected. (B) Western blot analysis of AR protein in the temporal cortex and cerebellum of AR97Q mice. (C) Densitometric quantitation of AR97Q band intensities shown in (B) (n = 4; ∗p < 0.05 two-sided t test). (D) Western blot analysis of AR protein in the temporal cortex and cerebellum of AR24Q mice. (E) Densitometric quantitation of AR24Q band intensities shown in (D) (n = 4). N.S., not significant. Error bars mean standard deviation.