TABLE 4.

Impact of spore monolayer versus spore multilayer in A. niger resistance to Mars-like conditions.

		Monolayer	Multilayer	f	p-value
Survival fraction a	Lab Control	5.3 × 10–1 ± 1.8 × 10–1	1.1 × 10–1 ± 5.9 × 10–2	↓	0.09
	UV shielded	6.4 × 10–1 ± 8.0 × 10–2	2.1 × 10–1 ± 6.6 × 10–2	↓	0.01*
	UV exposed	1.3 × 10–2 ± 4.5 × 10–3	2.1 × 10–1 ± 5.2 × 10–3	↑	0.5
Metabolic max. b	Lab Control	20% ± 5	23% ± 1	↑	0.6
	UV shielded	29% ± 1	36% ± 0.1	↑	0.002*
	UV exposed	13% ± 7	35% ± 0.5	↑	0.04*
Time until max. c	Lab Control	29 h ± 1.3	26 h ± 0.7	↓	0.1
	UV shielded	30 h ± 0.3	29 h ± 0.3	↓	0.07
	UV exposed	44 h ± 2	36 h ± 0.6	↓	0.002 *
Germination rated	Lab Control	0.38 ± 0.09	0.24 ± 0.03	↓	0.2
	UV shielded	0.47 ± 0.01	0.23 ± 0.04	↓	0.004 *
	UV exposed	0.01 ± 0.00	0.03 ± 0.01	↓	0.1

^aSurvival fraction calculated as N/N₀.

^bMetabolic activity maximum, measured as % of reduced resazurin.

^cIncubation time taken to reach maximum of metabolic activity (h).

^dTime of incubation until metabolic peak was detected (h).

^fPresence of spore multilayer has increased (↑) or decreased (↓) the measured parameter.