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. 2021 Feb 22;9:604400. doi: 10.3389/fcell.2021.604400

FIGURE 4.

FIGURE 4

LncRNA ANRIL acts as a sponge of miR-7-5p. (A) FISH assay showed the localization of lncRNA ANRIL in the cytoplasm. 18S and U6 were the internal control. (B) Transfection efficacy of miR-7-5p mimics and inhibitor was determined by qPCR. (C) Relative lncRNA ANRIL expression level was measured by qPCR in iPDLSCs after transfected with miR-7-5p mimics or miR-7-5p inhibitor. (C) FISH assay showed the localization of lncRNA ANRIL in the cytoplasm. 18S and U6 were the internal control. (D,E) The binding of miR-7-5p to ANRIL was validated by conducting the luciferase reporter assay. IPDLSCs were co-transfected with mimics-NC or miR-7-5p mimics and wild-type lncRNA ANRIL or mutant lncRNA ANRIL. Relative Renilla luciferase activity was normalized to that of firefly luciferase. Scale bar = 100 μm (*P < 0.05, **P < 0.01, ***P < 0.001).