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. 2020 Oct 9;28(3):952–967. doi: 10.1038/s41418-020-00627-5

Fig. 3. Autophagy inhibition promoted cell growth, migration, and invasion.

Fig. 3

DAPK3-overexpressed MKN45 cells were transfected with siRNAs against ATG5, ATG7, or control siRNA, respectively. Autophagy activity was examined by western blot assay (a) and GFP-LC3 fluorescence assay (b) (original magnification, 600×, scale bar: 20 μm). Cell growth rates, migration and invasion were measured by XTT assay (c), wound healing assay (d) and transwell assay (e) respectively (***P < 0.001, Student’s t test) (original magnification, 200×, scale bars: 100 μm).