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. 2020 Oct 28;28(3):1110–1125. doi: 10.1038/s41418-020-00640-8

Fig. 1. Single-cell profiling revealed cell heterogeneity in axolotl limb tissues from different regeneration stage.

Fig. 1

A Schematic diagram of the blastemal time course experiment. The red box on limbs indicated the amputation location on axolotls’ limbs (upper left). The pictures show that the amputated tissues were trypsinized to yield a single-cell suspension (upper middle) and trapped using the Fluidigm C1 auto prepare system (C1 high-throughput integrated fluidics circuits (HT IFCs) and HiSeq systems) for the downstream single-cell mRNA-seq experiment. B T-distributed stochastic neighbor embedding (t-SNE) visualizations of all cell clusters identified using the computational pipeline. C T- SNE visualizations of 4 days post amputation identified using the computational pipeline. D Heatmap of top 20 differentiated gene of seven clusters. E T-SNE distribution of cells from each sampling stage. Red dots represent the cells from the corresponding time point, and gray dots represent all the cells from four stages. F The proportion of cells at each time point in each cluster. G Violin plots of marker genes from seven cluster.