A t-SNE visualizations of cells at each postamputation stage. Colors and associated numbers represent individual clusters. t-SNE visualization of 0 dpa (B), 3 dpa (C), 7 dpa (D), and 21 dpa (E) scRNA-seq data overlaid with expression of the cell-type-specific markers COL2A1, COL1A2, COL1A1, THBS4, POSTN, and ACAN. Cells are color-coded according to expression level, ranging from low detected to the highest detected levels (gray, yellow, orange, and red). Cluster numbers are as shown in (A).