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. 2021 Feb 22;9:634242. doi: 10.3389/fcell.2021.634242

Figure 3.

Figure 3

MIG6 inhibits endothelial cell proliferation, migration, tube formation, and sprouting. (A) Western blot for MIG6 overexpression in HRECs treated with Ad-MIG6. (B) Representative images for EdU incorporation in MIG6-overexpressing HRECs. (C) Bar graphs represent the mean ± SEM of % EdU+ cells per field in (B). (D) Western blot for MIG6 expression in MIG6 knockdown HRECs. (E) Images showing EdU incorporation in MIG6 knockdown HRECs. (F) Bar graphs represent the mean ± SEM of % EdU+ cells per field in (E). (G) Tube formation in HRECs upon control (siControl) or MIG6 siRNA (siMIG6)-mediated knockdown. (H) Quantification of the number of the branch points per field in (G). (I) Representative images of EC spheroids using siMIG6 HREC for 48 h. (J) Quantification of the number of sprouts per EC spheroid in (I). (K) Quantification of the total sprout length per EC spheroid in (I). Scale bars: 50 μm for (B,E); 100 μm for (G); 500 μm for (I). The data are shown as mean ± SEM from three independent experiments. * p < 0.05, ** p < 0.01 (two-tailed paired Student's t-test).