Figure 6.

Co-infection of murine ODMs with T. gondii and G. duodenalis. Murine ODMs were infected with either an ID of 3 (G. duodenalis strain WB6 trophozoites) or an ID of 25 (T. gondii strain RH tachyzoites), or both and monitored for 48 h post infection. (A) Representative projections of immunofluorescence Z-stack images of Zo-1 and occludin of non-infected (NI), G. duodenalis, T. gondii and co-infected murine ODMs after 48 h. T. gondii’s mitochondrial GFP fluorescence was lost due to methanol fixation; instead, dashed lines indicate T. gondii vacuoles including parasite nuclei (blue). Scale bars represent 20 µm. All experiments were performed twice with similar results (B) TEER monitoring of infected murine ODMs. Data are presented as mean (± 95% CI) of four independent experiments with three filters per experiment. Statistical significance between infection and mock controls was determined using a Two-Way ANOVA with Dunnett’s correction for multiple testing. **p < 0.01, ****p < 0.0001 (C) Transcriptional changes of indicated tight junctional components in infected ODMs. Data are presented as mean (± 95% CI) of three independent experiments with three filters per experiment. Statistical significance was determined using a Two-Way ANOVA with Dunnett’s correction for multiple testing. *p < 0.05, ***p < 0.001.