Table 1.

Medium conditions for organoid culture.

Host	L-WRN CM*(50%)	Rspo1 CM**(20%)	Noggin CM***(10%)	EGF (50 ng/ml)	NAC (1 mM)	NIC(10 mM)	SB-202190 (1 μM)	A83-01 (500 nM)	Y-27632 (10 μM)	CHIR (3 μM)	PGE2 (10 μM)
Three-dimensional organoids
Mouse	X	X	X	Mouse	X	X	X	X
Human	X	X	X	Human	X	X	X	X
Pig	X	X	X	Mouse	X	X	X	X	X
Chicken	X	X	X	Human + Mouse	X	X	X	X	X	X	X
Organoid-derived monolayers
All	(Initially)	X	X	X	X	X			(Initially)

*L-WRN conditioned medium corresponds to 100 ng/ml recombinant Wnt3A.

**R-spondin1 conditioned medium corresponds to 1 µg/ml recombinant R-spondin.

***Noggin conditioned medium corresponds to 100 ng/ml recombinant Noggin.

NAC, N-acetylcysteine; NIC, nicotinamide.