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. 2021 Feb 22;12:628654. doi: 10.3389/fimmu.2021.628654

Figure 1.

Figure 1

Circ_0088194 is upregulated in RA-FLSs. (A) Hierarchical clustering analysis of differentially expressed CircRNAs between RA-FLSs and OA-FLSs. (B) QRT-PCR was used to detect the circRNA expression levels in nine RA-FLSs and seven OA-FLSs. (RA-FLSs = 9, OA-FLSs = 7). (C) Schematic illustration of how the Circ_0088194 is back-spliced from tenascin C (TNC) exons 17 to 21. The existence of Circ_0088194 was validated using qRT-PCR, followed by Sanger sequencing. (D) The TNC mRNA expression level, as detected using qRT-PCR in nine RA-FLSs and seven OA-FLSs. (RA-FLSs = 9, OA-FLSs = 7). (E) Divergent primers could amplify Circ_0088194 from cDNA but not from gDNA. (F) Circ_0088194 expression in RA-FLSs correlated positively with the RA disease activity score in 28 joints (DAS28 score). (n = 9; r = 0.683, P = 0.043). (G) Circ_0088194 showed a predominantly cytoplasmic localization upon RNA FISH. Scale bar, 500 µm. Data are shown as mean ± SD. NS, not significant. **indicates p < 0.01, and ****indicates p < 0.0001.