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. 2021 Feb 22;12:628654. doi: 10.3389/fimmu.2021.628654

Figure 3.

Figure 3

MMP2 expression in RA-FLSs is regulated by Circ_0088194. (A) Adenovirus expressing Circ_0088194 or empty vector (EV) were transfected into RA-FLSs. QRT-PCR determined tumor necrosis factor-α converting enzyme (TACE), platelet derived growth factor subunit AA (PDGF-AA), and matrix metalloproteinase 2 (MMP2) mRNA levels. (B) Adenovirus expressing Circ_0088194 or EV were transfected into RA-FLSs. Western blotting was used to detect MMP2 expression. (C, D) The MMP2 mRNA and protein expression levels were examined in RA-FLSs transfected with Circ_0088194#1 siRNAs or the negative control (NC). (E, F) Western blotting and qRT-PCR detection of MMP2 expression. RA-FLSs were co-transfected with the adenovirus expressing Circ_0088194 or MMP2 siRNA. (G, H) Transwell migration and Matrigel invasion assays were used to evaluate the migratory and invasive activity of RA-FLSs. Adenovirus expressing Circ_0088194 or MMP2 siRNA were co-transfected into RA-FLSs. Scale bar, 500 µm. Data are shown as mean ± SD. NS, not significant. *indicates p < 0.05, **indicates p < 0.01, and ***indicates p < 0.001.