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. 2021 Feb 22;9:623886. doi: 10.3389/fbioe.2021.623886

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Copyright © 2021 Zentelytė, Žukauskaitė, Jacerytė, Borutinskaitė and Navakauskienė.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell energy phenotype and metabolic alterations during treatments of small molecule combinations A and B on AFSCs. (A) Mitochondrial respiration and glycolytic activity expressed as normalized OCR and ECAR, respectively, of control and treated cells after the addition of ETC inhibitors oligomycin and FCCP. The time in the x-axis represents time points when each measurement was taken. (B) The relative expression of genes, related to cell metabolism and respiration: NRF1, HIF1α, PPARGC1A, ERRα, PKM, PDK1, and LDHA, (C) the relative expression of NF-κB family genes NFKB1, NFKB2, RELA, RELB, REL in control and treated cells as determined by using RT-qPCR. The data were normalized to GAPDH and presented as a fold change over untreated control. The data in A was obtained using Seahorse XFp Extracellular Flux Analyzer (Agilent, United States) and “Cell energy phenotype test.” Results are presented as mean ± SD. p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), where not indicated: non-significant.