Figure 2.

(A,B) Immunoblot and relative expression of CB₁R and endocannabinoid enzymes in hippocampal whole homogenates from WT and TRPV1^−/− mice. MAGL and FAAH increase significantly in TRPV1^−/−. Data were analyzed by means of non-parametric or parametric tests (Mann–Whitney U-test or Student's t-test). (C) Immunoblot of CB₁R in whole homogenates and P2 extracts of raw membranes from WT and TRPV1^−/− mice hippocampi. (D) Relative expression of CB₁R in P2 extracts from WT and TRPV1^−/−. Not significant changes are detected in TRPV1^−/−. Student's t-test. (E,F) Immunoblot and relative expression of CB₁R, endocannabinoid enzymes, and CB₁R interacting protein 1a in hippocampal synaptosomes from WT and TRPV1^−/− mice. CB₁R and MAGL decrease but CRIP1a increases significantly in TRPV1^−/−. Fisher's exact test. p > 0.05; p < 0.05*; p <0.005**; p< 0.001***. Data are expressed as mean ± S.E.M.