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. 2021 Feb 22;15:645940. doi: 10.3389/fnana.2021.645940

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Copyright © 2021 Egaña-Huguet, Bonilla-Del Río, Gómez-Urquijo, Mimenza, Saumell-Esnaola, Borrega-Roman, García del Caño, Sallés, Puente, Gerrikagoitia, Elezgarai and Grandes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specificity of the CB₁R antibodies in dentate ML of the CB₁R-KO mouse processed for electron microscopy. (A,C) Single pre-embedding immunogold method for CB₁R. (B,D) Double pre-embedding immunogold for CB₁R and immunoperoxidase for GFAP method. No CB₁R labeling is detected in the outer 2/3 (A,B) and inner 1/3 of the layer (C,D). Scale bars: 0.5 μm.