Figure 4.

CB₁R localization in the outer 2/3 ML of WT and TRPV1^−/− mice. Pre-embedding immunogold method for electron microscopy. CB₁R immunoparticles (arrows) are localized to inhibitory terminals (ter, red arrows, red shading), excitatory terminals (ter, green arrows, green shading), and mitochondrial outer membranes (m, purple arrows, purple shading) in WT (A,C) and TRPV1^−/− (B,D). CB₁R particles are also on membranes of GFAP positive astrocytic processes (as, yellow arrows, yellow shading) in WT (E) and TRPV1^−/− (F). Combined pre-embedding immunoperoxidase and immunogold method. sp, dendritic spine; den, dendrite; Scale bars: 0.5 μm.