Figure 5.

Statistical assessment of CB₁R labeling in several subcellular compartments in the outer 2/3 ML of WT and TRPV1^−/− mice. (A) Percentage of CB₁R positive excitatory and inhibitory terminals, mitochondria, and astrocytes. (B) Density of CB₁R (particles/μm). (C) Number of excitatory and inhibitory terminals, mitochondria, and astrocytes in 20 μm². (D) Perimeter (μm) of synaptic terminals. (E) Number of CB₁R positive excitatory and inhibitory terminals, mitochondria, and astrocytes in 20 μm². (F) Perimeter (μm) of CB₁R positive synaptic terminals. Data were analyzed by non-parametric or parametric tests (Mann–Whitney U-test or Student's t-test). (G) Proportion of CB₁R labeling in excitatory and inhibitory terminals, astrocytes, mitochondria (neuronal, astroglial), and dendrites normalized to the total CB₁R content. (H) Number of CB₁R particles in the compartments analyzed in (G). Chi square test. (I) Total number of CB₁R particles in 20 μm². (J) Number of CB₁R particles found in membranes in 20 μm². Mann–Whitney U-test or Student's t-test. p < 0.05*; p < 0.01**; p < 0.001***; p < 0.0001****. All data are represented as mean ± S.E.M.