Skip to main content
. 2021 Feb 22;11:609060. doi: 10.3389/fimmu.2020.609060

Figure 1.

Figure 1

TGR5 deficiency exacerbates steatohepatitis. WT and TGR5-/- mice were fed a normal or MCD diet for 6 weeks (n = 5/group). (A) Hepatic mRNA levels were examined by real-time quantitative PCR. (B, C) Protein expressions were examined by western blotting and relative density ratio of protein levels of TGR5 was analyzed (TGR5/β-actin). (D) Representative images of H&E and Oil-Red O in liver sections from mice (scale bar, 100 μm). (E) NAFLD activity scores were calculated, and steatosis, inflammation, and ballooning were increased in TGR5-/-–MCD mice. N.D., not detected. (F–H) Levels of serum ALT, AST, and hepatic triglycerides were increased in TGR5-/-–MCD mice. Data represent mean ± SD; *P < 0.05.