Fig. 6.

Mfn1 is a direct downstream mRNA of miR-26b-5p in CMECs. The predicted binding sites of Malat1 and miR-26b-5p and the mutated binding sites are indicated (A). Luciferase activities of reporter vectors containing luciferase genes and a fragment of Mfn1 RNA containing wild-type or mutated miR-26b-5p binding sites (B). Mfn1 was associated with miR-26b-5p. CMECs were transfected with biotinylated wild-type miR-26b-5p (Bio-miR-26b-5p-WT) or biotinylated mutant miR-26b-5p (Bio-miR-26b-5p-Mut) (C). Concentration gradients of the miR-26b-5p mimic and inhibitor were transfected into CMECs, and quantitative analysis of the alteration of miR-26b-5p and the gene and protein expression of Mfn1 was performed (D-E). *p < 0.05 compared with the NC group, #p < 0.05 compared with the adjacent group. n = 5 in each group.