Skip to main content
. 2021 Feb 22;41:101910. doi: 10.1016/j.redox.2021.101910

Fig. 8.

Fig. 8

Mfn1 reversed mitochondrial damage and apoptosis induced by silencing Malat1 in CMECs under hypoxia. Relative protein expression of Mfn1 in CMECs (A). Relative cell viability was measured by the CCK-8 assay (B). Nitric oxide (NO) content in CMECs (C). Cellular ROS (green) and mtROS (red) in CMECs were measured and quantified (D). Mitochondria were stained with MitoTracker (red), and mitochondrial morphology was quantified (E). The expression of mitochondrial fusion/fission-related proteins and mitophagy-related proteins in CMECs (F). Mitochondrial membrane potential (ΔΨm) was measured by the JC-1 assay (G). TUNEL staining of CMECs and the percentage of TUNEL-positive CMECs (red) were quantified (H). The expression of apoptosis-associated proteins in CMECs (I, J). *p < 0.05 compared with the siNC + pcDNA3.1 group, #p < 0.05 compared with the hy + siNC + pcDNA3.1 group, & p < 0.05 compared with the hy + siMalat1+pcDNA3.1 group. n = 5 in each group.