Figure 5.

Mpeg1::mCherry reporters facilitate the tracking of macrophages in larval zebrafish. Because the expression of Perforin-2 is largely restricted to macrophages in zebrafish, the insertion of mCherry into the Mpeg1 locus allows localization of macrophages in both fixed and live animals. The transgenic animals above were infected with sublethal and lethal doses of Shigella flexneri and fixed 24 h post infection. The above images have been previously published (58) and are a composite of the red and green fluorescent channels. This figure was adapted from source images posted at https://doi.org/10.1371/journal.ppat.1003588.g002 under the CC BY 4.0 license.