Figure 1.

Chenodeoxycholic acid (CDCA), but not recombinant FGF19, downregulated bile acids (BA) synthesis in primary human hepatocytes. (A, B) CYP7A1 mRNA expression (24 h, n = 13; 6 h, n = 10) and (C, D) cholic acid (CA) concentration (24 h, n = 5; 6 h, n = 5) in cell medium following treatment with FGF19 or CDCA at various concentrations for 24 h or 6 h. Data is presented as box-plot showing interquartile range (IQR) (box) and min-max (bars) with median marked with a line, red dots are the individual values, black dots in (A) represents RNA from donors that were also used for RNA sequencing. The plus sign represents mean value. Friedman test was used to assess differences between control and treatments and Dunn’s multiple comparison test was used post hoc. *p < 0.05, **p < 0.01, ***p < 0.001.