Figure 3.

Medium containing FGF19 produced by primary human hepatocytes downregulated CYP7A1 mRNA expression. (A) Layout of the experiment showing how chenodeoxycholic acid (CDCA) was used to induce FGF19 synthesis in primary human hepatocytes. Endogenously produced FGF19 was then applied to naïve hepatocytes for 24 h. (B) Levels of FGF19 in control and conditioned medium. (C) CYP7A1 mRNA expression and (D) cholic acid (CA) concentration in control and conditioned medium. Data is presented as box-plot showing interquartile range (IQR) (box) and min-max (bars) with median marked with a line, marked with red dots are the individual values, black dots in (C) represents RNA from donors that were also used for RNA sequencing. (n = 10). The plus sign represents mean value. Wilcoxon matched-pairs signed rank test was used to assess differences between control and conditioned medium. *p < 0.05, ns: non significant.