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. 2021 Feb 22;9:630361. doi: 10.3389/fcell.2021.630361

FIGURE 2.

FIGURE 2

Expression of Kir5.1 in the WT mouse cochlea. (A) Immunofluorescence staining with antibodies against Kir5.1 (red), Myo7A (green), and Sox2 (blue) and DAPI staining (white) in the basal turn of the mouse cochlea at P90. There was no difference in the immunolabeling of Kir5.1 from the apical to basal turns. Scale bar = 10 μm. (B) Immunohistochemical staining with antibodies against Kir5.1 (red) and Myo7A (green) and DAPI staining (white). Scale bar = 50 μm. (C) Immunofluorescence staining with antibodies against Kir5.1 (red) and DAPI staining (white) in the cochlea at P3, P8, P10, P12, P14, and P21. Scale bar = 10 μm. (D) Q-PCR results showing the changes in Kcnj16 mRNA in the mouse cochlea from P3 to P21. GAPDH was used as the internal control. Primers are shown in the Supplementary Table 2. Data are presented as the mean ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001, n = 4. OHC, outer hair cell; IHC, inner hair cell; SC, supporting cell; SL, spiral ligament; II, IV, V, type II, IV, and V fibrocytes in the spiral ligament. Lim, spiral limbus; SG, spiral ganglions.