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. 2021 Feb 22;9:630361. doi: 10.3389/fcell.2021.630361

FIGURE 4.

FIGURE 4

Cochlear development and stereocilia structures were normal in the Kcnj16− /− mice. (A) Auditory HCs of P30 and P120 mice were stained with antibodies against Myo7A and imaged using a confocal microscope. Images were taken from the basal turn of the cochlea. There was no difference in the staining from the apical to basal turns. Scale bar = 10 μm. (B) The HCs were counted and compared with age-matched WT mice (p > 0.05, n = 6). Data are presented as the mean ± SD. (C) Auditory HC stereocilia of Kcnj16− /− and WT mice were stained with phalloidin, and images were taken from the basal turn of the cochlea. There was no difference from the apical to basal turns. Scale bar = 10 μm. (D) Low magnification and high magnification scanning electron microscope images of OHC stereocilia bundles in Kcnj16− /− and WT mice. Images were taken from the middle turn of P60 mice. Scale bar = 5 μm. (E) Auditory HCs of P3 and P14 mice were stained with FM1-43, and images were taken from the basal turns. There was no difference in the immunolabeling signals from the apical to basal turns (data not shown). Scale bar = 10 μm.