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. 2021 Feb 22;10:600762. doi: 10.3389/fonc.2020.600762

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Copyright © 2021 Zhu, Sun, Sun, Chen, Wu and Hou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MLK3 regulates the localization of EPS8 in U251 and U118 cells. (A, B) The MAP3K11 gene knockout increases the expression of EPS8 in U251 (A) and U118 (B) cells. Relative levels of MLK3 were normalized to U251 wt or U118 wt groups. GAPDH was used as a loading control. Two-tailed Student’s t-test. n = 3, *P < 0.05, **P < 0.01. AU, arbitrary unit. (C, D) The MAP3K11 gene knockout alters the location of EPS8 in U251 (C) and U118 (D) cells. The results shown are representative of at least three independent cultures. EPS8 (green); DAPI-stained nuclei (blue). Scale bars, 20 μm.