Fig 7.

In vivo verification of immune peptide/proteins of fraction B against P. larvae infection. I: Survivorship of control and treated larval groups (50 larvae each) throughout a larval duration period (five days). G1: negative control group fed on a normal diet (ND), G2: positive control group fed on an ND + peptides of fraction B, G3: infected group fed on an ND + P. larvae spores, and G4: treated group fed on an ND + P. larvae spores + peptides of fraction B. Mortality rates were calculated daily in triplicate (n = 3). Lines marked with similar letters represent no significant differences (P > 0.05); While different letters represent significant differences (P ≤ 0.05; One-way ANOVA). II: One-dimensional SDS-PAGE of plasma from P. larvae-injected (Pi) and non-injected control (C) larvae. Each lane was loaded with 2.5 µL of plasma (pooled from five individual larvae). M: Standard marker reference proteins (kDa). Infected plasma clearly shows different protein over-expression compared with non-injected controls.