Table 3.
Declared and analyzed α-amylase activity in the experimental diets.
| Diets | Amylase, KNU1/kg |
|
|---|---|---|
| Declared | Analyzed | |
| Corn + Soybean meal | 0 | <LOD |
| Corn + Soybean meal | 40 | 32 |
| Corn + Soybean meal | 80 | 53 |
| Corn + Soybean meal | 120 | 70 |
| Corn + Soybean meal | 160 | 141 |
| Corn + Soybean Meal +40% Corn | 0 | <LOD |
| Corn + Soybean Meal +40% Corn | 40 | 44 |
| Corn + Soybean Meal +40% Corn | 80 | 65 |
| Corn + Soybean Meal +40% Corn | 120 | 127 |
| Corn + Soybean Meal +40% Corn | 160 | 151 |
Abbreviation: LOD = limit of detection.
1
Kilo novo α-amylase units is the amount of enzyme that releases 5.26 g of starch per h in a two-step reaction, 6 μmol p-nitrophenol per min from 1.86 mM ethyledene-G7-p-nitrophenyl-maltoheptaoside at pH 7.0 and 37°C.