Fig. 3.

Psma2-O/E and -shRNA increased and decreased cell proliferation and tumour volume respectively under high glucose environment Cell viability (a) and proliferation (b) were measured in CUP-1 cells with and without psma2-O/E in normal or high glucose medium by MTT and cell counting. The upregulation of the RNA and protein expression of psma2 was confirmed after enforced overexpression by RT-PCR and WB (e, f). Cell viability (c) and proliferation (d) were measured in CUP-1 cells with and without psma2-shRNA in normal or high glucose medium by MTT and WB. Downregulation of the RNA expression of psma2 was confirmed after psma2-shRNA transfection by RT-PCR and WB (g, h). (e-f) Tumour volume was measured twice weekly after inoculation of CUP-1 cells with psma2-shRNA or vector into db/db mice (N = 7 in each group) (i, j). Cell proliferation marker Ki67 (k, l) was measured in the tumour of the above diabetes/cancer mice (red: Ki67, blue: DAPI, magnification: × 200. The white scale bar represents 50 μm). Data are presented as mean±SD with individual data points in histograms. * p < 0.05, ** p < 0.01, *** p < 0.001. (For interpretation of the references to color in this figure legend, reader can refer to the web version of this article.)