Table 5.
RT enzyme and priming strategies used for norovirus NGS studies.
| Paper | Reverse transciptase enzymes | Priming strategy |
|---|---|---|
| Bartsch et al., 2018* | Not provided | Not provided |
| Bavelaar et al., 2015 | REPLI-g sc Polymerase | Random hexamers and oligo dT primers |
| Boonchan et al., 2017 | Qiagen One Step RT PCR enzyme (Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase) | Not provided |
| Brown et al., 2016 | SuperScript III | Random hexamers |
| Casto et al., 2018 | SuperScript III | Random hexamers |
| Chan et al., 2017 | SuperScript III | Tagged random octamers |
| Chen et al., 2018 | Ovation RNA sequencing (RNA-Seq) system version 2 kit (NuGen, USA) | Chimeric primer mix |
| Chhabra et al., 2018 | Not provided | In-house degenerate primer |
| Cotten et al., 2014a | SuperScript III | Tiling approach custom primer panel |
| Cotten et al., 2014b | SuperScript III | Endoh et al. (2005) hexamers |
| Cuevas et al., 2016 | SuperScript III, AccuScript Hi-Fi reverse transcription (Agilent) | Random hexamers, custom primer |
| Fonager et al., 2017 | SMARTer RNA stranded sequencing kit | Oligo(dT) |
| Fumian et al., 2019* | High Capacity cDNA Reverse Transcription Kit and MultiScribeTM Reverse Transcriptase (Thermo Fisher) | Random primers |
| Hasing et al., 2016 | TruSeq RNA sample preparation kit v2 with SuperScript II | Oligo(dT) |
| Imamura et al., 2016a* | High Capacity cDNA Reverse Transcription Kit and MultiScribeTM Reverse Transcriptase (Thermo Fisher) | Oligo(dT) |
| Imamura et al., 2016b* | High Capacity cDNA Reverse Transcription Kit and MultiScribeTM Reverse Transcriptase (Thermo Fisher) | Oligo(dT) |
| Imamura et al., 2017* | High Capacity cDNA Reverse Transcription Kit and MultiScribeTM Reverse Transcriptase (Thermo Fisher) | Oligo(dT) |
| Kundu et al., 2013 | SuperScript III | Random hexamers |
| Nasheri et al., 2017 | TruSeq Stranded mRNA SuperScript III | Random hexamers |
| Strubbia et al., 2019a* | SuperScript II | Random hexamers |
| Strubbia et al., 2019b | SuperScript II, SuperScript III | Non-ribosomal hexamers (Endoh et al., 2005), random hexamers |
| Strubbia et al., 2020* | SuperScript II | Random hexamers (New England Biolabs (NEB), USA), I-HD hexamers, non-ribosomal hexamers (Endoh et al., 2005) |
| Suffredini et al., 2018* | MyTaqTM One-Step RT-PCR Kit | Target specific primers |
| van Beek et al., 2017 | SuperScript III | Random hexamers |
*
Environmental application.