Table 7.

Literature investigating the impact of RT enzyme on cDNA fidelity.

Paper	RT enzymes	Main findings
Waugh et al., 2015	SuperScript II and AMV RT enzymes	RNA concentration during first strand synthesis; effect of RNase H activity and PCR cycling conditions all impact both the yield and fidelity of RT
		Input RNA concentration and PCR cycles might generate a larger viral cDNA population for analysis but are likely to compromise the quality of sequencing data obtained
Yasukawa et al., 2017	HIV-1 RT, AMV, or MMLV	HIV-1 RT demonstrated lower fidelity than AMV or MMLV
Okano et al., 2018	HIV-1 RT, AMV, or MMLV	High concentrations of MgCl2 and dNTP negatively impact RT fidelity
Zucha et al., 2020	Maxima H, SuperScript II, Superscript IV, PrimeScript, SensiScript, Accuscript	Performance reproducibility was best for Maxima H and Superscript IV
Cholet et al., 2020	SuperScript II, SuperScript IV, Sesniscript, Omnicsript,	Addition of RNA mock communities into environmental RNA (before reverse transcription) can aid interpret sequencing results