Table A1.

Overview of primers used in this study (utr., untranslated). Please note that the nitroreductase GlNR1 is annotated as Fd‐NR2, that GlNR2 is annotated as Fd‐NR1, and that GlNR3 is annotated as nitroreductase family protein in the Giardia data base (http://giardiadb.org/giardiadb/). The coding sequences are underlined. ADI, arginine deiminase (GL50803_112103)

Name	Sequence	Gene (accession number)	Use
NR3_F	CACCATGGTTGAAGGTTATCCTG	Nitroreductase family protein GlNR3 (GL50803_15307)	Cloning into pET‐His151 for expression in Escherichia coli
NR3_R	TTACTCTATCATATAAATTCGTC		Idem
NR3_fusion_F	GAGTCGACCCTCCCATTTGTGACGCT		Introduction of a HincII‐site at the 5'‐end of NR3.
NR3_fusion_R	GAGAGCTCTTACTCTATCATATAAATTCGTC		Introduction of a SacI‐site at the 3'‐end of NR3, both for cloning into pET‐His151 containing the GlNR2 coding sequence.
GlNR1_ADI_F	GATCTAGAAACGTCTACACGTGAGGTGTGTAAACTTCCGGAGAAAAAAATCCTAGTACATGGTTGAAGGTTATCCTG	Fd‐NR2 GlNR1 (GL50803_22677)	Introduction of a XbaI‐site and of the ADI promotor at the 5'‐end of NR1.
GlNR1_ADI_R	GATTAATTAACTGGATATGAACATGTCAATTATTTGATATCTGAATTACAATTCACTGTTTTACTTAAATGTAATGTCGAC		Introduction of a PacI‐site and of the ADI‐downstream region at the 3'‐end of NR1.
GlNR2_ADI_F	GAGCTAGCAACGTCTACACGTGAGGTGTGTAAACTTCCGGAGAAAAAAATCCTAGTACATGTCACGCTTTCCAGAGG	Fd‐NR1 GlNR2 (GL50803_6175)	Introduction of a NheI‐site and of the ADI promotor at the 5'‐end of NR2.
GlNR2_ADI_R	GATTAATTAACTGGATATGAACATGTCAATTATTTGATATCTGAATTACAATTCACTGTTTTATTCCACAAACGTTACGTCTATCTTAAATGTAATGTCGAC		Introduction of a PacI‐site and of the ADI‐downstream region at the 3'‐end of NR2.
GlNR3_ADI_F	GATCTAGAAACGTCTACACGTGAGGTGTGTAAACTTCCGGAGAAAAAAATCCTAGTACATGCCTCCCATTTGTGAC		Introduction of a XbaI‐site and of the ADI promotor at the 5'‐end of NR3.
GlNR2_ADI_R	GATTAATTAACTGGATATGAACATGTCAATTATTTGATATCTGAATTACAATTCACTGTTTTATTCCACAAACGTTACGTCTATCTTAAATGTAATGTCGAC		Introduction of a PacI‐site and of the ADI‐downstream region at the 3'‐end of NR3.