Figure 3. Deletion of MAK3 impairs growth on glycerol and sodium chloride.

(A) Schematic of the experimental setup. BY4741 WT and mak3∆ mutant strains transformed with an empty vector or plasmid expressing HA-NAA30 were ten-fold serial diluted, spotted on to SD-Ura plates with various stressors and incubated at 30°C, if not indicated otherwise. (B) The plates were incubated for 2 days for 30°C, 3 days for 300 mM CaCl₂, 0.1% (w/v) caffeine (5.15 mM) and 75 mM HU, 4 days for 37°C and 1 M NaCl, and 5 days for 15°C and 2% glycerol. All experiments were performed three times. Representative results are shown.