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. 2021 Mar 4;41(3):BSR20202828. doi: 10.1042/BSR20202828

Figure 6. Human NAA30 rescues growth defect of mak3∆ cells in glycerol medium.

Figure 6

WT (BY4741) and mak3Δ mutant cells transformed with empty pBEVY-U vector or HA-NAA30 plasmid were grown overnight in SD-Ura medium. Cells were washed and resuspended in sterile water. Liquid growth assays were performed by culturing the yeast cells in 96-well plate at 30°C in (A) SD-Ura or (B) SDAll medium with 2% glucose or 3% glycerol as sole carbon source. Yeast growth was monitored by measuring absorbance at 595 nm every 15 min using a microplate reader. (C) Growth data were fitted with logistic non-linear regression growth model. The relative growth rates (k) are shown as bar graphs with SE indicated. Student’s t-test was performed to determine the significant differences in growth rates between the WT strain and mak3∆ strains using the individual rate constants (k) (*P≤0.05; ***P≤0.001; n=3).